| RK | LN | Name | Time | Gap | FINA | Q? |
|---|---|---|---|---|---|---|
| 1 | 4 | Nagy Anna Zsófia (2008) UNI Győri Úszó Sportegy. | 00:35.99R:0.5 | - | 531 | |
| 2 | 5 | Straková Katarína (2010)VŠK FTVŠ UK Lafranconi | 00:37.35R:0.87 | 00:01.36 | 475 | |
| 3 | 6 | Mills Brisa Anne (2007)Pápai Úszó SE | 00:39.64R:0.71 | 00:03.65 | 398 | |
| 4 | 2 | Tamás Rebeka (2011) UNI Győri Úszó Sportegy. | 00:40.26R:0.68 | 00:04.27 | 379 | |
| 5 | 3 | Májeková Tereza (2010)VŠK FTVŠ UK Lafranconi | 00:40.35R:0.73 | 00:04.36 | 377 | |
| 6 | 9 | Göbl-Fürnkranz Emma (2009)USV Krems | 00:40.57R:0.67 | 00:04.58 | 371 | |
| 7 | 0 | Horváth Emma Sára (2007)Szombathelyi SK SI | 00:40.60R:0.73 | 00:04.61 | 370 | |
| 8 | 1 | Brevák Szonja (2011)Soproni Széchy T. SI | 00:40.75R:0.79 | 00:04.76 | 366 | |
| 9 | 8 | Varga Hanga (2011) UNI Győri Úszó Sportegy. | 00:42.37R:0.73 | 00:06.38 | 325 | |
| 10 | 7 | Stéger Georgina (2012)DELFIN SE | 00:42.71R:0.9 | 00:06.72 | 318 |