| RK | Name | H/L | Time | Gap | Q |
|---|---|---|---|---|---|
| 1 | Szabała Antonina (2009)POLISH SWIMMING FEDERATION | 01:01.12 | - | ||
| 2 | Leśniewska Barbara (2010)POLISH SWIMMING FEDERATION | 01:02.98 | 01.86 | ||
| 3 | Kertész Boróka (2010)HUNGARIAN SWIMMING FEDERATION | 01:03.35 | 02.23 | ||
| 4 | Rékasi Zsuzsanna (2009)HUNGARIAN SWIMMING FEDERATION | 01:03.70 | 02.58 | ||
| 5 | Martišovičová Nella (2009)SLOVAK SWIMMING FEDERATION | 01:04.24 | 03.12 | ||
| 6 | Sladkova Barbora (2009)CZECH SWIMMING FEDERATION | 01:06.01 | 04.89 | ||
| 7 | Klohnová Ella (2010)SLOVAK SWIMMING FEDERATION | 01:09.84 | 08.72 | ||
| 8 | Zatloukalova Ema (2010)CZECH SWIMMING FEDERATION | 01:10.56 | 09.44 | ||
| - | Kiraľová Kristína (2010)SLOVAK SWIMMING FEDERATION | OOC | |||
| - | Zelena Barbora (2010)CZECH SWIMMING FEDERATION | OOC | |||