| RK | Name | H/L | Time | Gap | Q |
|---|---|---|---|---|---|
| 1 | Takács Botond (2009)HUNGARIAN SWIMMING FEDERATION | 00:27.92 102.480% | - | ||
| 2 | Nawojczyk Michał (2009)POLISH SWIMMING FEDERATION | 00:28.15 100.389% | 00:00.23 | ||
| 3 | Hynek Oliver (2009)CZECH SWIMMING FEDERATION | 00:28.71 100.243% | 00:00.79 | ||
| 4 | Bozsodi Máté (2010)HUNGARIAN SWIMMING FEDERATION | 00:29.02 101.124% | 00:01.10 | ||
| 5 | Musil Frantisek (2010)CZECH SWIMMING FEDERATION | 00:29.14 104.082% | 00:01.22 | ||
| 6 | Kajan Maxim (2009)SLOVAK SWIMMING FEDERATION | 00:29.20 102.210% | 00:01.28 | ||
| 7 | Kwiatkowski Jan (2010)POLISH SWIMMING FEDERATION | 00:29.77 100.568% | 00:01.85 | ||
| 8 | Čamaj Ján (2010)SLOVAK SWIMMING FEDERATION | 00:32.58 - | 00:04.66 | ||