| RK | Name | H/L | Time | Gap | Q |
|---|---|---|---|---|---|
| 1 | Stawicki Mateusz (2009)POLISH SWIMMING FEDERATION | 51.57 | - | ||
| 2 | Pápai Olivér (2009)HUNGARIAN SWIMMING FEDERATION | 51.94 | 00.37 | ||
| 3 | Lebeda Stepan (2009)CZECH SWIMMING FEDERATION | 54.38 | 02.81 | ||
| 4 | MACHÁŇ Leonard (2009)SLOVAK SWIMMING FEDERATION | 54.43 | 02.86 | ||
| 5 | Hołub Hubert (2010)POLISH SWIMMING FEDERATION | 55.04 | 03.47 | ||
| 6 | Nagy Péter (2010)HUNGARIAN SWIMMING FEDERATION | 57.10 | 05.53 | ||
| 7 | Solin Petr (2010)CZECH SWIMMING FEDERATION | 57.41 | 05.84 | ||
| 8 | Hornák Sebastián (2010)SLOVAK SWIMMING FEDERATION | 57.88 | 06.31 | ||
| - | Nemes Ármin Boldizsár (2009)HUNGARIAN SWIMMING FEDERATION | OOC | |||
| - | Tarasiewicz Piotr (2009)POLISH SWIMMING FEDERATION | OOC | |||
| - | Trydulski Wiktor (2010)POLISH SWIMMING FEDERATION | OOC | |||