| RK | Name | H/L | Time | Gap | Q |
|---|---|---|---|---|---|
| 1 | Szabała Antonina (2009)POLISH SWIMMING FEDERATION | 00:27.27 100.365% | - | ||
| 2 | Leśniewska Barbara (2010)POLISH SWIMMING FEDERATION | 00:28.62 102.487% | 00:01.35 | ||
| 3 | Kertész Boróka (2010)HUNGARIAN SWIMMING FEDERATION | 00:28.69 100.623% | 00:01.42 | ||
| 4 | Zavacká Andrea (2009)SLOVAK SWIMMING FEDERATION | 00:28.96 100.172% | 00:01.69 | ||
| 5 | Rékasi Zsuzsanna (2009)HUNGARIAN SWIMMING FEDERATION | 00:29.07 99.377% | 00:01.80 | ||
| 6 | Sladkova Barbora (2009)CZECH SWIMMING FEDERATION | 00:29.19 101.218% | 00:01.92 | ||
| 7 | Krištofíková Simona (2010)SLOVAK SWIMMING FEDERATION | 00:29.27 101.348% | 00:02.00 | ||
| 8 | Zatloukalova Ema (2010)CZECH SWIMMING FEDERATION | 00:30.86 98.487% | 00:03.59 | ||
| - | Duda Natalia (2010)POLISH SWIMMING FEDERATION | OOC 100.534% | |||
| - | Klohnová Ella (2010)SLOVAK SWIMMING FEDERATION | OOC - | |||
| - | Zverinova Melanie (2010)CZECH SWIMMING FEDERATION | OOC 103.448% | |||