| RK | Name | H/L | Time | Gap | Q |
|---|---|---|---|---|---|
| 1 | Leśniewska Barbara (2010)POLISH SWIMMING FEDERATION | 26.55 98.857% | - | ||
| 2 | Gil Laura (2009)POLISH SWIMMING FEDERATION | 26.77 100.705% | 00.22 | ||
| 3 | Szabó Kincső Hanna (2010)HUNGARIAN SWIMMING FEDERATION | 26.86 100.445% | 00.31 | ||
| 4 | Tombor Sára (2009)HUNGARIAN SWIMMING FEDERATION | 26.90 100.518% | 00.35 | ||
| 5 | Zavacká Andrea (2009)SLOVAK SWIMMING FEDERATION | 27.39 99.634% | 00.84 | ||
| 6 | Krištofíková Simona (2010)SLOVAK SWIMMING FEDERATION | 27.64 100.253% | 01.09 | ||
| 7 | Zatloukalova Ema (2010)CZECH SWIMMING FEDERATION | 27.80 100.394% | 01.25 | ||
| 8 | Blazkova Alzbeta (2009)CZECH SWIMMING FEDERATION | 28.84 101.063% | 02.29 | ||
| - | Dudek Lena (2009)POLISH SWIMMING FEDERATION | OOC 100.180% | |||
| - | Sujka Ewa (2009)POLISH SWIMMING FEDERATION | OOC 98.585% | |||
| - | Zverinova Melanie (2010)CZECH SWIMMING FEDERATION | OOC 102.935% | |||