| RK | Name | H/L | Time | Gap | Q |
|---|---|---|---|---|---|
| 1 | Minta Zofia (2009)POLISH SWIMMING FEDERATION | 33.36 99.579% | - | ||
| 2 | Megyesi Vanda (2009)HUNGARIAN SWIMMING FEDERATION | 33.39 98.418% | 00.03 | ||
| 3 | Danielova Barbora (2009)CZECH SWIMMING FEDERATION | 33.62 100.679% | 00.26 | ||
| 4 | Puzsa Petra (2010)HUNGARIAN SWIMMING FEDERATION | 34.17 101.186% | 00.81 | ||
| 5 | Wołącewicz Natalia (2010)POLISH SWIMMING FEDERATION | 34.97 100.484% | 01.61 | ||
| 6 | Brabcova Barbora (2010)CZECH SWIMMING FEDERATION | 35.29 99.830% | 01.93 | ||
| 7 | Daňová Noemi (2010)SLOVAK SWIMMING FEDERATION | 36.06 97.992% | 02.70 | ||
| - | Szaturska Emilia (2009)POLISH SWIMMING FEDERATION | OOC 103.189% | |||
| - | Lecká Dominika (2009)SLOVAK SWIMMING FEDERATION | DSQ - | |||