| RK | LN | Name | Time | Gap | FINA | Q? |
|---|---|---|---|---|---|---|
| 1 | 4 | Tóth Laura (2007)Hullám 91 | 39.89R:0.71 | - | 396 | |
| 2 | 2 | Fábián Laura (2012)A Jövő SC | 41.86R:0.59 | 01.97 | 342 | |
| 3 | 5 | Alt Kamilla Emma (2011)Budapesti Honvéd | 41.98 | 02.09 | 339 | |
| 4 | 7 | Nyári Hanna (2012)Budaörsi Sport Club | 42.95R:0.66 | 03.06 | 317 | |
| 5 | 3 | Rakaczky Villő (2012)Darnyi Tamás SC | 43.93R:0.56 | 04.04 | 296 | |
| 6 | 6 | Holló Ida Franciska (2010)Budapesti Honvéd | 44.02R:0.61 | 04.13 | 294 | |
| 7 | 0 | Dániel Réka (2012)DELFIN SE | 47.04R:0.99 | 07.15 | 241 | |
| 8 | 8 | Madarassy Borbála (2012)Darnyi Tamás SC | 48.23R:0.59 | 08.34 | 224 | |
| 9 | 9 | Sólyom Karolin Kata (2013)Iron Swim SE | 48.44R:0.72 | 08.55 | 221 | |
| - | 1 | Simon Júlia (2011)Bohóchal Egyesület | DNS |