| RK | LN | Name | Time | Gap | FINA | Q? |
|---|---|---|---|---|---|---|
| 1 | 4 | Suijker Rachel (2012)Váci Vízmű SE | 36.75 | - | 506 | |
| 2 | 5 | Fábián Laura (2012)A Jövő SC | 39.83R:0.6 | 03.08 | 398 | |
| 3 | 3 | Konkoly Lilla Dóra (2012)Aquaworm Waterpolo | 41.50R:0.73 | 04.75 | 351 | |
| 4 | 7 | Gosztonyi Eszter (2012)Iron Swim SE | 42.05R:0.69 | 05.30 | 338 | |
| 5 | 2 | Hegedűs Lilla (2012)Budaörsi Sport Club | 42.50R:0.64 | 05.75 | 327 | |
| 6 | 6 | Filetóth Szonja Zoé (2012)Darnyi Tamás SC | 42.98R:0.58 | 06.23 | 316 | |
| 7 | 0 | Nagy Réka (2012)Budapesti Honvéd | 44.89R:0.76 | 08.14 | 278 | |
| 8 | 1 | Morvay Veronika Lilla (2012)Kőbánya Sport Club | 45.01R:0.98 | 08.26 | 275 | |
| 9 | 8 | Pichovszky Zita (2012)Budaörsi Sport Club | 45.14R:0.79 | 08.39 | 273 | |
| 10 | 9 | Madarassy Borbála (2012)Darnyi Tamás SC | 45.43R:0.51 | 08.68 | 268 |