| RK | LN | Name | Time | Gap | FINA | Q? |
|---|---|---|---|---|---|---|
| 1 | 5 | Takács Botond (2009)HUNGARIAN SWIMMING FEDERATION | 00:27.92R:0.62 | - | 600 | |
| 2 | 4 | Nawojczyk Michał (2009)POLISH SWIMMING FEDERATION | 00:28.15R:0.6 | 00:00.23 | 585 | |
| 3 | 3 | Hynek Oliver (2009)CZECH SWIMMING FEDERATION | 00:28.71R:0.62 | 00:00.79 | 551 | |
| 4 | 6 | Bozsodi Máté (2010)HUNGARIAN SWIMMING FEDERATION | 00:29.02R:0.65 | 00:01.10 | 534 | |
| 5 | 1 | Musil Frantisek (2010)CZECH SWIMMING FEDERATION | 00:29.14R:0.67 | 00:01.22 | 527 | |
| 6 | 2 | Kajan Maxim (2009)SLOVAK SWIMMING FEDERATION | 00:29.20R:0.72 | 00:01.28 | 524 | |
| 7 | 7 | Kwiatkowski Jan (2010)POLISH SWIMMING FEDERATION | 00:29.77R:0.58 | 00:01.85 | 495 | |
| 8 | 8 | Čamaj Ján (2010)SLOVAK SWIMMING FEDERATION | 00:32.58R:0.68 | 00:04.66 | 377 |