| RK | LN | Name | Time | Gap | FINA | Q? |
|---|---|---|---|---|---|---|
| 1 | 4 | Spiering Femke (2002)ZPC AMERSFOORT | 00:26.04R:0.71 | - | 745 | |
| 2 | 5 | Boerefijn Nynke (2007)ZPC AMERSFOORT | 00:27.44R:0.79 | 00:01.40 | 636 | |
| 3 | 3 | Gonda Zita (2008)Hód Úszó SE | 00:27.74R:0.63 | 00:01.70 | 616 | |
| 4 | 6 | Metten Fenne (2010)ZPC AMERSFOORT | 00:28.07R:0.41 | 00:02.03 | 595 | |
| 5 | 2 | Varga Nóra (2011)Bálinti Úszó Egyes. | 00:28.41R:0.71 | 00:02.37 | 573 | |
| 6 | 7 | Varga Tímea (2010)Bálinti Úszó Egyes. | 00:28.81R:0.7 | 00:02.77 | 550 | |
| 7 | 1 | Balázs Éva (2010)Debreceni Aquasport | 00:29.00R:0.54 | 00:02.96 | 539 | |
| 8 | 9 | Cserhalmi Anna Lili (2012)Debreceni Aquasport | 00:29.67 | 00:03.63 | 503 | |
| 9 | 8 | Lénárt Zsófia Irén (2012)Hód Úszó SE | 00:29.78R:0.68 | 00:03.74 | 498 | |
| 10 | 0 | Egbrink Renske Oude (2006)OZ&PC | 00:29.87R:0.71 | 00:03.83 | 493 |