| RK | LN | Name | Time | Gap | FINA | Q? |
|---|---|---|---|---|---|---|
| 1 | 5 | Minta Zofia (2009)POLISH SWIMMING FEDERATION | 33.36R:0.68 | - | 667 | |
| 2 | 4 | Megyesi Vanda (2009)HUNGARIAN SWIMMING FEDERATION | 33.39R:0.71 | 00.03 | 666 | |
| 3 | 3 | Danielova Barbora (2009)CZECH SWIMMING FEDERATION | 33.62R:0.79 | 00.26 | 652 | |
| 4 | 6 | Puzsa Petra (2010)HUNGARIAN SWIMMING FEDERATION | 34.17R:0.74 | 00.81 | 621 | |
| 5 | 2 | Wołącewicz Natalia (2010)POLISH SWIMMING FEDERATION | 34.97R:0.73 | 01.61 | 579 | |
| 6 | 7 | Brabcova Barbora (2010)CZECH SWIMMING FEDERATION | 35.29R:0.78 | 01.93 | 564 | |
| 7 | 1 | Daňová Noemi (2010)SLOVAK SWIMMING FEDERATION | 36.06R:0.8 | 02.70 | 528 | |
| - | 8 | Lecká Dominika (2009)SLOVAK SWIMMING FEDERATION | DSQ |